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PLoS One. 2012;7(11):e49723. doi: 10.1371/journal.pone.0049723. Epub 2012 Nov 16.

Activated human T cells secrete exosomes that participate in IL-2 mediated immune response signaling.

Author information

1
University of Gothenburg, Department of Rheumatology and Inflammation Research, Gothenburg, Sweden.

Abstract

It has previously been shown that nano-meter sized vesicles (30-100 nm), exosomes, secreted by antigen presenting cells can induce T cell responses thus showing the potential of exosomes to be used as immunological tools. Additionally, activated CD3⁺ T cells can secrete exosomes that have the ability to modulate different immunological responses. Here, we investigated what effects exosomes originating from activated CD3⁺ T cells have on resting CD3⁺ T cells by studying T cell proliferation, cytokine production and by performing T cell and exosome phenotype characterization. Human exosomes were generated in vitro following CD3⁺ T cell stimulation with anti-CD28, anti-CD3 and IL-2. Our results show that exosomes purified from stimulated CD3⁺ T cells together with IL-2 were able to generate proliferation in autologous resting CD3⁺ T cells. The CD3⁺ T cells stimulated with exosomes together with IL-2 had a higher proportion of CD8⁺ T cells and had a different cytokine profile compared to controls. These results indicate that activated CD3⁺ T cells communicate with resting autologous T cells via exosomes.

PMID:
23166755
PMCID:
PMC3500321
DOI:
10.1371/journal.pone.0049723
[Indexed for MEDLINE]
Free PMC Article

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