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Philos Trans R Soc Lond B Biol Sci. 2013 Jan 5;368(1609):20110329. doi: 10.1098/rstb.2011.0329.

Recent advancements in cloning by somatic cell nuclear transfer.

Author information

1
RIKEN BioResource Center, Tsukuba, Ibaraki, Japan. ogura@rtc.riken.go.jp

Abstract

Somatic cell nuclear transfer (SCNT) cloning is the sole reproductive engineering technology that endows the somatic cell genome with totipotency. Since the first report on the birth of a cloned sheep from adult somatic cells in 1997, many technical improvements in SCNT have been made by using different epigenetic approaches, including enhancement of the levels of histone acetylation in the chromatin of the reconstructed embryos. Although it will take a considerable time before we fully understand the nature of genomic programming and totipotency, we may expect that somatic cell cloning technology will soon become broadly applicable to practical purposes, including medicine, pharmaceutical manufacturing and agriculture. Here we review recent progress in somatic cell cloning, with a special emphasis on epigenetic studies using the laboratory mouse as a model.

PMID:
23166393
PMCID:
PMC3539358
DOI:
10.1098/rstb.2011.0329
[Indexed for MEDLINE]
Free PMC Article

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