Format

Send to

Choose Destination
See comment in PubMed Commons below
Chembiochem. 2012 Dec 21;13(18):2722-8. doi: 10.1002/cbic.201200525. Epub 2012 Nov 20.

Programming in situ immunofluorescence intensities through interchangeable reactions of dynamic DNA complexes.

Author information

1
Departments of Bioengineering and Chemistry, Rice University, Houston, Texas 77030, USA.

Abstract

The regulation of antibody reporting intensities is critical to various in situ fluorescence-imaging analyses. Although such control is often necessary to visualize sparse molecular targets, the ability to tune marker intensities is also essential for highly multiplexed imaging strategies in which marker reporting levels must be tuned both to optimize dynamic detection ranges and to minimize crosstalk between different signals. Existing chemical amplification approaches generally lack such control. Here, we demonstrate that linear and branched DNA complexes can be designed to function as interchangeable building blocks that can be assembled into organized, fluorescence-reporting complexes. We show that the ability to program DNA-strand-displacement reactions between these complexes offers new opportunities to deterministically tune the number of dyes that are coupled to individual antibodies in order both to increase and controllably balance marker reporting levels within fixed cells.

PMID:
23165916
PMCID:
PMC3715551
DOI:
10.1002/cbic.201200525
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Support Center