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Mitochondrion. 2013 Jan;13(1):7-14. doi: 10.1016/j.mito.2012.11.001. Epub 2012 Nov 16.

Mitochondrial respiration in human viable platelets--methodology and influence of gender, age and storage.

Author information

1
Mitochondrial Pathophysiology Unit, Lund University, 221 84 Lund, Sweden. fredrik.sjovall@med.lu.se

Abstract

Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.

PMID:
23164798
DOI:
10.1016/j.mito.2012.11.001
[Indexed for MEDLINE]
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