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Anal Chem. 2012 Dec 18;84(24):10645-9. doi: 10.1021/ac302236a. Epub 2012 Nov 30.

Ultrasensitive immunoassay based on anodic near-infrared electrochemiluminescence from dual-stabilizer-capped CdTe nanocrystals.

Author information

1
School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China.

Abstract

A sandwich-typed near-infrared (NIR) electrochemiluminescence (ECL) immunoassay was developed with dual-stabilizer-capped CdTe nanocrystals (NCs) as ECL labels and α fetoprotein antigen (AFP) as model protein. The dual-stabilizer-capped NIR CdTe NCs were promising ECL labels because of their NIR ECL emission of 800 nm, low anodic ECL potential of +0.85 V, and high biocompatibity, which can facilitate interference-free and highly sensitive ECL bioassays. Upon the immunorecognition of the immobilized AFP to its antibody labeled with dual-stabilizer-capped CdTe NCs, the proposed immunoassay displayed increasing ECL intensity, leading to a wide calibration range of 10.0 pg/mL to 80.0 ng/mL with a detection limit of 5.0 pg/mL [signal-to-noise ratio (S/N) = 3] without coupling any signal amplification procedures. The NIR ECL immunoassay for real samples displayed very similar results with those of Ru(bpy)(3)(2+) reagent kit based commercial ECL immunoassay, which not only proved for the efficiency of NIR ECL from dual-stabilizer-capped CdTe NCs but also paved the road for development of novel ECL emitters and corresponding reagent kits.

PMID:
23163822
DOI:
10.1021/ac302236a
[Indexed for MEDLINE]

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