Format

Send to

Choose Destination
See comment in PubMed Commons below
Inorg Chem. 2012 Dec 3;51(23):12591-3. doi: 10.1021/ic301305q. Epub 2012 Nov 19.

A naked-eye on-off-on molecular "light switch" based on a reversible "conformational switch" of G-quadruplex DNA.

Author information

  • 1Department of Chemistry, Tongji University, Shanghai 200092, People's Republic of China.

Abstract

Herein, we report a new strategy for developing an on-off-on molecular "light switch" by utilizing the pH value to control the "conformational switch" of G-quadruplex DNA. A novel ruthenium(II) complex with an emission enhancement factor of 150 was synthesized and introduced to detect the switch by the naked eye. The "light switch" can be repeatedly cycled off and on through the addition of H(+) and OH(-), respectively. The conformational transitions of G-quadruplex DNA in K(+) solution at different pH values in the acidic region were evidenced by circular dichroism and fluorescence titrations. Computational calculations by applying density functional theory (DFT)/time-dependent DFT and molecular docking were also carried out to gain insight into the "light-switch" mechanism.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Write to the Help Desk