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Biomed Opt Express. 2012 Nov 1;3(11):2752-60. doi: 10.1364/BOE.3.002752. Epub 2012 Oct 3.

Optical clearing of archive-compatible paraffin embedded tissue for multiphoton microscopy.

Author information

  • 1Department of Chemistry, Duke University, Durham, NC 27708, USA.

Erratum in

  • Biomed Opt Express. 2013 Feb;4(2):219.

Abstract

Standard histopathology techniques (including paraffin embedding) are incompatible with thick tissue multiphoton imaging, and standard clearing techniques on those specimens destroy some molecular information. We demonstrate multiphoton imaging in specimens prepared according to standard histopathology techniques. This permits unlabeled 3-dimensional histology on archival tissue banks, which is of great value in evaluating prognostic indicators.

KEYWORDS:

(110.0113) Imaging through turbid media; (170.6900) Three-dimensional microscopy; (170.6930) Tissue

PMID:
23162713
PMCID:
PMC3493235
DOI:
10.1364/BOE.3.002752
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