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Macromol Biosci. 2012 Dec;12(12):1627-36. doi: 10.1002/mabi.201200262. Epub 2012 Nov 19.

In vitro 3D full-thickness skin-equivalent tissue model using silk and collagen biomaterials.

Author information

1
Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.

Abstract

Current approaches to skin equivalents often only include the epidermis and dermis. Here, a full-thickness skin equivalent is described including epidermis, dermis, and hypodermis, that could serve as an in vitro model for studying skin biology or as a platform for consumer product testing. The construct is easy to handle and is maintained for >14 d while expressing physiological morphologies of the epidermis and dermis, seen by keratin 10, collagens I and IV expression. The skin equivalent produces glycerol and leptin, markers of adipose metabolism. This work serves as a foundation for understanding a few necessary factors needed to develop a stable, functional model of full-thickness skin.

PMID:
23161763
PMCID:
PMC3724336
DOI:
10.1002/mabi.201200262
[Indexed for MEDLINE]
Free PMC Article

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