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Angew Chem Int Ed Engl. 2013 Jan 7;52(2):749-53. doi: 10.1002/anie.201204645. Epub 2012 Nov 19.

In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies.

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1
Patterning and Fabrication, Institute of Materials Research and Engineering, Agency for Science, Technology and Research, Singapore, Singapore.

Abstract

The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd(22)-PEO(13) and PMOXA(20)-PDMS(54)-PMOXA(20) block copolymer vesicles. The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening.

PMID:
23161746
DOI:
10.1002/anie.201204645
[Indexed for MEDLINE]
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