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Toxicol Ind Health. 2014 Nov;30(10):910-8. doi: 10.1177/0748233712464809. Epub 2012 Nov 16.

The protective role of pomegranate juice against carbon tetrachloride-induced oxidative stress in rats.

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Chemistry Department, University of Dicle, Diyarbakır, Turkey.
Chemistry Department, University of Dicle, Diyarbakır, Turkey
Physiology Department, Faculty of Veterinary Science, University of Dicle, Diyarbakır, Turkey.
Histology and Embryology Department, Faculty of Veterinary Science, University of Dicle, Diyarbakır, Turkey.


Most pomegranate (Punica granatum Linn., Punicaceae) fruit parts are known to possess enormous antioxidant activity. The present study was carried out to determine the phenolic and flavonoid contents of Derik pomegranate juice and determine its effect against carbon tetrachloride (CCl4)-induced toxicity in rats. Animals were divided into four groups (n = 6): group I: control, group II: CCl4 (1 ml/kg), group III: CCl4 + pomegranate juice and group IV: CCl4 + ursodeoxycholic acid (UDCA). Treatment duration was 4 weeks, and the dose of CCl4 was administered once a week to groups II, III and IV during the experimental period. CCl4-treated rats caused a significant increase in serum enzyme levels, such as aspartate aminotransferase, alanine aminotransferase and total bilirubin, and decrease in albumin, when compared with control. Administration of CCl4 along with pomegranate juice or UDCA significantly reduces these changes. Analysis of lipid peroxide (LPO) levels by thiobarbutiric acid reaction showed a significant increase in liver, kidney and brain tissues of CCl4-treated rats. However, both pomegranate juice and UDCA prevented the increase in LPO level. Histopathological reports also revealed that there is a regenerative activity in the liver and kidney cells. Derik pomegranate juice showed to be hepatoprotective against CCl4-induced hepatic injury. In conclusion, present study reveals a biological evidence that supports the use of pomegranate juice in the treatment of chemical-induced hepatotoxicity.


Pomegranate juice; antioxidant activity; carbon tetrachloride toxicity; lipid peroxidation; ursodeoxycholic acid

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