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Cell Cycle. 2012 Dec 1;11(23):4385-9. doi: 10.4161/cc.22712. Epub 2012 Nov 16.

Presence of a defect in karyokinesis during megakaryocyte endomitosis.

Author information

1
INSERM, UMR 1009, Institut Gustave Roussy, Villejuif, France.

Abstract

Megakaryocyte is the naturally polyploid cell that gives rise to platelets. Polyploidization occurs by endomitosis, a process corresponding to a late failure of cytokinesis with a backward movement of the daughter cells. Generally, a pure defect in cytokinesis produces a multinucleated cell, but megakaryocytes are characterized by a single polylobulated nucleus with a 2 (N) ploidy. Here, we show the existence of a defect in karyokinesis during the endomitotic process. From late telophase until the reversal of cytokinesis, some dipolar mitosis/endomitosis and most multipolar endomitosis present a thin DNA link between the segregated chromosomes surrounded by an incomplete nuclear membrane formation, which implies that sister chromatid separation is not complete. This observation may explain why polyploid megakaryocytes display a single polylobulated nucleus along with an increase in ploidy.

PMID:
23159853
PMCID:
PMC3552921
DOI:
10.4161/cc.22712
[Indexed for MEDLINE]
Free PMC Article

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