Format

Send to

Choose Destination
PLoS One. 2012;7(11):e48604. doi: 10.1371/journal.pone.0048604. Epub 2012 Nov 14.

Multi-state proteins: approach allowing experimental determination of the formation order of structure elements in the green fluorescent protein.

Author information

1
Institute of Protein Research, RAS, Pushchino, Moscow Region, Russia.

Abstract

The most complex problem in studying multi-state protein folding is the determination of the sequence of formation of protein intermediate states. A far more complex issue is to determine at what stages of protein folding its various parts (secondary structure elements) develop. The structure and properties of different intermediate states depend in particular on these parts. An experimental approach, named μ-analysis, which allows understanding the order of formation of structural elements upon folding of a multi-state protein was used in this study. In this approach the same elements of the protein secondary structure are "tested" by substitutions of single hydrophobic amino acids and by incorporation of cysteine bridges. Single substitutions of hydrophobic amino acids contribute to yielding information on the late stages of protein folding while incorporation of ss-bridges allows obtaining data on the initial stages of folding. As a result of such an μ-analysis, we have determined the order of formation of beta-hairpins upon folding of the green fluorescent protein.

PMID:
23155397
PMCID:
PMC3498258
DOI:
10.1371/journal.pone.0048604
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center