(a) One Hundred Springs Plain Spring (OSP) located within Norris Geyser Basin (YNP). Arrows indicate spring source and sampling points with corresponding temperature and dissolved oxygen values obtained along primary flow path. (b) NWF-PCA of four replicate de novo assemblies for NAG1 (circled, four shades of brown and red) and genomes from T. pendens, Candidatus Korarchaeum cryptofilum, N. maritimus and F. acidarmanus. Axes x, y, z correspond to PC1, PC2 and PC3. (c) Average G+C content (%) of NAG1 scaffolds from replicate OSPB-1 (red circles) plotted as a function of decreasing scaffold length (solid black line, cumulative total sequence length; solid red line, average G+C content; dotted line, indicates first eight scaffolds where consensus sequence is >90%).