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Pharm Biol. 2013 Mar;51(3):350-60. doi: 10.3109/13880209.2012.729067. Epub 2012 Nov 15.

Anti-inflammatory action of Tamarind seeds reduces hyperglycemic excursion by repressing pancreatic β-cell damage and normalizing SREBP-1c concentration.

Author information

1
Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, University of Delhi, New Delhi, India. sush.sole@gmail.com

Abstract

CONTEXT:

Tamarindus indica L. (Leguminosae) is widely used as a traditional medicine for the management of diabetes mellitus (DM) in India, in addition to its anti-inflammatory activity. The present study has been designed to understand the correlation involved between antidiabetic and anti-inflammatory action of aqueous seed extract of T. indica (TSE) in diabetic rats.

OBJECTIVE:

In view of the fact that fatty acid synthesis and insulin release from islets of pancreas are regulated by sterol regulatory element-binding proteins (SREBP-1c) and cytosolic calcium, respectively, the objectives of present study were to determine the influence of TSE on SREBP-1c mRNA and to investigate the intracellular islets calcium [Ca²⁺](I) involvement and β-cell mass preservation in insulin secretagogue action of TSE.

MATERIALS AND METHODS:

The effect of 4 weeks oral treatment (120 and 240 mg/kg) of high-performance liquid chromatography (HPLC) standardized TSE was studied in streptozotocin (STZ)-induced diabetic male Wistar rats. Reverse transcription-PCR (RT-PCR) and a spectrofluorometer were used for mRNA concentration and islets [Ca²⁺](I) determination, respectively. The TUNEL assay was followed to study the pancreatic apoptosis.

RESULTS:

TSE (120 and 240 mg/kg) showed positive correlation with [Ca²⁺](I) and insulin release. The anti-inflammatory action of TSE was significant on nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in addition to a favorable effect on β-cell neogenesis and improved mRNA concentration of SREBP-1c.

DISCUSSION AND CONCLUSION:

The results suggest that anti-inflammatory action of Tamarind seeds on β-cell cells of islets and cytokines contribute toward its antidiabetic activity by way of complex mechanisms of [Ca²⁺](I) handling and through SREBP-1c gene in liver.

PMID:
23151094
DOI:
10.3109/13880209.2012.729067
[Indexed for MEDLINE]

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