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Int J Cancer. 2013 Jun 1;132(11):2578-88. doi: 10.1002/ijc.27936. Epub 2013 Feb 8.

Cell-based selection provides novel molecular probes for cancer stem cells.

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1
Department of Chemistry, University of Florida, Gainesville, FL, USA.

Abstract

Cancer stem cells (CSC) represent a malignant subpopulation of cells in hierarchically organized tumors. They constitute a subpopulation of malignant cells within a tumor mass and possess the ability to self-renew giving rise to heterogeneous tumor cell populations with a complex set of differentiated tumor cells. CSC may be the cause of metastasis and therapeutic refractory disease. Because few markers exist to identify and isolate pure CSC, we used cell-based Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX) to create DNA aptamers that can identify novel molecular targets on the surfaces of live CSC. Out of 22 putative DNA sequences, 3 bound to ~90% and 5 bound to ~15% of DU145 prostate cancer cells. The 15% of cells that were positive for the second panel of aptamers expressed high levels of E-cadherin and CD44, had high aldehyde dehydrogenase 1 activity, grew as spheroids under nonadherent culture conditions, and initiated tumors in immune-compromised mice. The discovery of the molecular targets of these aptamers could reveal novel CSC biomarkers.

PMID:
23150432
PMCID:
PMC4115063
DOI:
10.1002/ijc.27936
[Indexed for MEDLINE]
Free PMC Article
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