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Methods Mol Biol. 2013;935:207-13. doi: 10.1007/978-1-62703-080-9_14.

Detection of DNA fragmentation in retinal apoptosis by TUNEL.

Author information

1
Tumour Biology Laboratory, Biochemistry Department, Bioscience Research Institute, University College Cork, Cork, Ireland.

Abstract

Terminal dUTP nick end labeling (TUNEL) is an invaluable technique used in the study of late-stage apoptosis. The technique is based upon detection of fragmented DNA, a well-recognized characteristic of apoptosis, usually with fluorescent markers. Here, we describe the TUNEL technique (1) employing two different detection techniques, fluorescence microscopy and fluorescence-activated cell sorting (2) which can be applied to the analysis of apoptosis in retinal tissues or retinal cell cultures, respectively.

PMID:
23150370
DOI:
10.1007/978-1-62703-080-9_14
[Indexed for MEDLINE]

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