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Mem Inst Oswaldo Cruz. 2012 Nov;107(7):960-3.

An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y.

Author information

1
Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brasil. alexsantos@ioc.fiocruz.br

Abstract

The detection of anti-hepatitis A virus (HAV) antibody levels by diagnostic kits in the convalescent period of disease generally use immunoglobulin G (IgG), which is expensive. An alternative to IgG is immunoglobulin Y (IgY), an immunoglobulin antibody encountered in birds and reptiles. The aim of this study was to develop a competitive immunoenzymatic assay to measure total anti-HAV antibody levels using anti-HAV IgY as the capture and conjugated immunoglobulins. For this purpose, anti-HAV IgY was conjugated to horseradish peroxidase (HRP) and the optimal dilution of HRP-conjugated antibodies was evaluated to establish the competitive immuneenzymatic assay. The results obtained from our "in-house" assay were plotted on a receiver operator curve, which showed a sensitivity of 95% and a specificity of 98.8%, demonstrating that a competitive anti-HAV IgY immunoenzymatic assay developed "in house" could be used as an alternative to commercial assays that utilise IgG.

PMID:
23147158
DOI:
10.1590/s0074-02762012000700022
[Indexed for MEDLINE]
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