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Mem Inst Oswaldo Cruz. 2012 Nov;107(7):935-9.

Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil.

Author information

1
Departamento de Medicina Veterinária Preventiva, Laboratório de Virologia Animal, Universidade Estadual de Londrina, PR, Brasil. no_rovaris@yahoo.com.br

Abstract

The objective of this study was to detect and identify hepatitis E virus (HEV) strains in liver and bile samples from slaughtered pigs in the state of Paraná, Brazil. Liver and bile samples were collected from 118 asymptomatic adult pigs at a slaughterhouse in a major Brazilian pork production area. The samples were assayed using a nested reverse transcription-polymerase chain reaction protocol with primer sets targeting open reading frames (ORF)1 and 2 of the HEV genome. HEV RNA was detected in two (1.7%) liver samples and one (0.84%) bile sample using both primers sets. The HEV strains were classified as genotype 3b on the basis of their nucleotide sequences. These data suggest that healthy pigs may be a source of HEV infection for consumers of pig liver and slaughterhouse workers in Brazil.

PMID:
23147152
[Indexed for MEDLINE]
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