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Asian Pac J Trop Med. 2012 Nov;5(11):897-900. doi: 10.1016/S1995-7645(12)60167-9.

Influence of Ginkgo biloba extract on the proliferation, apoptosis of ACC-2 cell and Survivin gene expression in adenoid cystic carcinoma of lacrimal gland.

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1
Department of Ophthalmology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Abstract

OBJECTIVE:

To explore the influence of extract of Ginkgo biloba (EGB) on the proliferation, apoptosis of ACC-2 cell and Survivin gene expression in adenoid cystic carcinoma (ACC) of lacrimal gland.

METHODS:

ACC-2 cell in human with ACC of lacrimal gland was in vitro cultured. MTT method was used for cell proliferation detection. Annexin V/PI double-staining flow cytometer was used to detect cell apoptosis and cell cycle. Survivin gene expression was analyzed by RT-PCR and Western blotting.

RESULTS:

EGB had inhibitory effect on the proliferation of ACC-2 cell with significant dose-effect relationship, and there was statistical difference when compared with the control group (P<0.01). The inhibitory concentration 50 % (IC(50)) is 88 mg/L. The flow cytometer test indicated that EGB can gradually increase ACC-2 cell in G(0)-G(1) stage and decrease it in G(2)-M and S stage. With the increase of dose, the apoptosis rate of ACC-2 cell was obviously increased (P<0.05 or P<0.01). EGB had certain inhibitory effect on Survivin gene expression of ACC-2 cell, and Survivin gene expression was decreased with the increasing of the EGB concentration (P<0.01).

CONCLUSIONS:

EGB can effectively inhibit Survivin gene expression of ACC-2 cell in human with ACC of lacrimal gland, induce the apoptosis of ACC-2 cell and inhibit tumor cell proliferation.

PMID:
23146805
DOI:
10.1016/S1995-7645(12)60167-9
[Indexed for MEDLINE]
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