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Anal Biochem. 2013 Mar 1;434(1):52-3. doi: 10.1016/j.ab.2012.10.042. Epub 2012 Nov 9.

Single-step cloning of a hybridoma producing a monoclonal antibody against a target protein.

Author information

  • 1Department of Life Science, University of Hyogo, Akou-gun, Hyogo 678-1297, Japan. orii@sci.u-hyogo.ac.jp

Abstract

We have developed a simple and time-saving method to identify hybridoma clones producing an antibody against a target protein among a large number of hybridomas in a single step. This method is very useful as the primary screening tool for hybridomas producing monoclonal antibodies if several micrograms of the target protein is available.

PMID:
23146588
DOI:
10.1016/j.ab.2012.10.042
[PubMed - indexed for MEDLINE]
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