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Chem Commun (Camb). 2012 Dec 28;48(100):12192-4. doi: 10.1039/c2cc36404b.

Simple and sensitive fluorescence detection of the RNA endonuclease activity of mammalian argonaute2 protein based on an RNA molecular beacon.

Author information

1
College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Shandong Normal University, Jinan 250014, China.

Abstract

A new strategy for determining the RNA endonuclease activity of mammalian argonaute2 (Ago2) protein has been developed, which combines the unique cleavage function of Ago2 protein with an RNA molecular beacon (RMB). Through the fluorescence restoration of the RMB, simple and sensitive detection of Ago2 is achieved.

PMID:
23145433
DOI:
10.1039/c2cc36404b
[Indexed for MEDLINE]

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