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PLoS One. 2012;7(11):e48582. doi: 10.1371/journal.pone.0048582. Epub 2012 Nov 7.

Suprabasin is hypomethylated and associated with metastasis in salivary adenoid cystic carcinoma.

Author information

1
Department of Otolaryngology-Head and Neck Surgery, the Johns Hopkins Medical Institutions, Baltimore, MD, USA.

Abstract

BACKGROUND:

Salivary gland adenoid cystic carcinoma (ACC) is a rare cancer, accounting for only 1% of all head and neck malignancies. ACC is well known for perineural invasion and distant metastasis, but its underlying molecular mechanisms of carcinogenesis are still unclear.

PRINCIPAL FINDINGS:

Here, we show that a novel oncogenic candidate, suprabasin (SBSN), plays important roles in maintaining the anchorage-independent and anchorage-dependent cell proliferation in ACC by using SBSN shRNA stably transfected ACC cell line clones. SBSN is also important in maintaining the invasive/metastatic capability in ACC by Matrigel invasion assay. More interestingly, SBSN transcription is significantly upregulated by DNA demethylation induced by 5-aza-2'-deoxycytidine plus trichostatin A treatment and the DNA methylation levels of the SBSN CpG island located in the second intron were validated to be significantly hypomethylated in primary ACC samples versus normal salivary gland tissues.

CONCLUSIONS/SIGNIFICANCE:

Taken together, these results support SBSN as novel oncogene candidate in ACC, and the methylation changes could be a promising biomarker for ACC.

PMID:
23144906
PMCID:
PMC3492451
DOI:
10.1371/journal.pone.0048582
[Indexed for MEDLINE]
Free PMC Article

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