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Nat Methods. 2012 Dec;9(12):1192-4. doi: 10.1038/nmeth.2225. Epub 2012 Nov 11.

Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.

Author information

1
Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.

Abstract

We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.

PMID:
23142872
PMCID:
PMC3863600
DOI:
10.1038/nmeth.2225
[Indexed for MEDLINE]
Free PMC Article

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