Format

Send to

Choose Destination
Biochem Biophys Res Commun. 2012 Dec 14;429(3-4):142-7. doi: 10.1016/j.bbrc.2012.10.125. Epub 2012 Nov 6.

Hippocalcin mediates calcium-dependent translocation of brain-type creatine kinase (BB-CK) in hippocampal neurons.

Author information

1
Department of Physiology, Toho University School of Medicine, Ohmori-nishi 5-21-16, Ohta-ku, Tokyo 143-8540, Japan.

Abstract

Hippocalcin (Hpca) is a Ca(2+)-binding protein that is expressed in neurons and contributes to neuronal plasticity. We purified a 48 kDa Hpca-associated protein from rat brain and identified it to be the creatine kinase B (CKB) subunit, which constitutes brain-type creatine kinase (BB-CK). Hpca specifically bound to CKB in a Ca(2+)-dependent manner, but not to the muscle-type creatine kinase M subunit. The N-terminal region of Hpca was required for binding to CKB. Hpca mediated Ca(2+)-dependent partial translocation of CKB (approximately 10-15% of total creatine kinase activity) to membranes. N-myristoylation of Hpca was critical for membrane translocation, but not for binding to CKB. In cultured hippocampal neurons, ionomycin treatment led to colocalization of Hpca and CKB adjacent to the plasma membrane. These results indicate that Hpca associates with BB-CK and that together they translocate to membrane compartments in a Ca(2+)-dependent manner.

PMID:
23142228
DOI:
10.1016/j.bbrc.2012.10.125
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center