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Bioengineered. 2013 Jul-Aug;4(4):249-53. doi: 10.4161/bioe.22818. Epub 2012 Nov 8.

Potential of Ophiostoma piceae sterol esterase for biotechnologically relevant hydrolysis reactions.

Author information

1
Centro de Investigaciones Biológicas CIB, Spanish National Research Council CSIC Madrid, Spain.

Abstract

The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, and the hydrolytic activity of the recombinant protein (OPE*) turned out to be improved from a kinetic point of view. In this study, we analyze the effects of sorbitol during the expression of OPE*, at first added as an additional carbon source, and methanol as inducer. The O. piceae enzyme was successfully used for PVAc hydrolysis, suggesting its potential applicability in recycled paper production to decrease stickies problems.

KEYWORDS:

Pichia pastoris; methanol; polyvinyl acetate; recombinant protein; recycled paper; sorbitol; stickies

PMID:
23138020
PMCID:
PMC3728196
DOI:
10.4161/bioe.22818
[Indexed for MEDLINE]
Free PMC Article
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