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Mol Nutr Food Res. 2013 Apr;57(4):698-708. doi: 10.1002/mnfr.201200069. Epub 2012 Nov 7.

Plasma B vitamins and LINE-1 DNA methylation in leukocytes of patients with a history of colorectal adenomas.

Author information

1
Department of Epidemiology, Biostatistics, and HTA, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands.

Abstract

SCOPE:

Low concentrations of folate, other B vitamins, and methionine are associated with colorectal cancer risk, possibly by changing DNA methylation patterns. Here, we examine whether plasma concentrations of B vitamins and methionine are associated with methylation of long interspersed nuclear element-1 (LINE-1) among those at high risk of colorectal cancer, i.e. patients with at least one histologically confirmed colorectal adenoma (CRA) in their life.

METHODS AND RESULTS:

We used LINE-1 bisulfite pyrosequencing to measure global DNA methylation levels in leukocytes of 281 CRA patients. Multivariable linear regression was used to assess associations between plasma B vitamin concentrations and LINE-1 methylation levels. Plasma folate was inversely associated with LINE-1 methylation in CRA patients, while plasma methionine was positively associated with LINE-1 methylation.

CONCLUSION:

This study does not provide evidence that in CRA patients, plasma folate concentrations are positively related to LINE-1 methylation in leukocytes but does suggest a direct association between plasma methionine and LINE-1 methylation in leukocytes.

PMID:
23132835
DOI:
10.1002/mnfr.201200069
[Indexed for MEDLINE]
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