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Methods Mol Biol. 2013;955:461-74. doi: 10.1007/978-1-62703-176-9_24.

High-resolution imaging of 2D outer membrane protein F crystals by atomic force microscopy.

Author information

1
Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland.

Abstract

In this chapter the methodological bases are provided to achieve subnanometer resolution on two-dimensional (2D) membrane protein crystals by atomic force microscopy (AFM). This is outlined in detail with the example of AFM studies of the outer membrane protein F (OmpF) from the bacterium Escherichia coli (E. coli). We describe in detail the high-resolution imaging of 2D OmpF crystals in aqueous solution and under near-physiological conditions. The topographs of OmpF, and stylus effects and artifacts encountered when imaging by AFM are discussed.

PMID:
23132075
DOI:
10.1007/978-1-62703-176-9_24
[Indexed for MEDLINE]

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