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Methods Mol Biol. 2013;955:297-305. doi: 10.1007/978-1-62703-176-9_16.

Automated grid handling and image acquisition for two-dimensional crystal screening.

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1
Department of Cell Biology, The National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, CA, USA. acheng@scripps.edu

Abstract

Routine large-scale two-dimensional (2D) crystallization trials are good candidates for automation. For imaging the large number of grids prepared from 2D crystallization trials, a two-pass imaging protocol using Leginon and a grid handling robot is described. A manual target selection bridges the two imaging passes. The two passes can be combined into one if objects of interests at different stages of trials can be reliably found using automated methods.

PMID:
23132067
DOI:
10.1007/978-1-62703-176-9_16
[Indexed for MEDLINE]
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