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Methods Mol Biol. 2013;955:129-52. doi: 10.1007/978-1-62703-176-9_8.

Recording high-resolution images of two-dimensional crystals of membrane proteins.

Author information

1
The WM Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA.

Abstract

Principles underlying the recording of high-quality/resolution images of two-dimensional crystals of membrane proteins are discussed in the context of instrumental conditions and operational procedures. A detailed example of low-dose microscope settings is provided along with an overview of a program that implements a computer-aided data acquisition procedure.

PMID:
23132059
DOI:
10.1007/978-1-62703-176-9_8
[Indexed for MEDLINE]

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