Synthesis and fluorescence study of a quaternized copolymer containing pyrene for DNA-hybridization detection

Lingye Yang; Min Zhao; Ruichen Zhang; Jie Dong; Ting Zhang; Xiaowei Zhan; Guojie Wang

doi:10.1002/cphc.201200765

Synthesis and fluorescence study of a quaternized copolymer containing pyrene for DNA-hybridization detection

Chemphyschem. 2012 Dec 21;13(18):4099-104. doi: 10.1002/cphc.201200765. Epub 2012 Nov 5.

Authors

Lingye Yang¹, Min Zhao, Ruichen Zhang, Jie Dong, Ting Zhang, Xiaowei Zhan, Guojie Wang

Affiliation

¹ School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China.

PMID: 23129170
DOI: 10.1002/cphc.201200765

Abstract

Built-in detector: A quaternized copolymer containing pyrene is described, which can bind to single-stranded DNA by electrostatic interactions to form a novel complex probe for the detection of DNA hybridization through strengthened fluorescence quenching through its intercalation into double-stranded DNA (see figure).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrylates / chemistry
Base Pair Mismatch
Biosensing Techniques / methods
DNA / chemistry*
Fluorescence
Methacrylates / chemical synthesis*
Methacrylates / chemistry
Molecular Probe Techniques
Nucleic Acid Hybridization / methods*
Oligonucleotide Probes / chemistry
Pyrenes / chemistry*
RNA / chemistry
Sensitivity and Specificity

Substances

Acrylates
Methacrylates
Oligonucleotide Probes
Pyrenes
RNA
DNA
pyrene
2-(dimethylamino)ethyl methacrylate