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J Cell Biol. 2012 Nov 12;199(4):685-98. doi: 10.1083/jcb.201206064. Epub 2012 Nov 5.

Drosophila PATJ supports adherens junction stability by modulating Myosin light chain activity.

Author information

  • 1Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, Germany.

Erratum in

  • J Cell Biol. 2013 Mar 18;200(6):853.

Abstract

The assembly and consolidation of the adherens junctions (AJs) are key events in the establishment of an intact epithelium. However, AJs are further modified to obtain flexibility for cell migration and morphogenetic movements. Intact AJs in turn are a prerequisite for the establishment and maintenance of apical-basal polarity in epithelial cells. In this study, we report that the conserved PDZ (PSD95, Discs large, ZO-1) domain-containing protein PATJ (Pals1-associated tight junction protein) was not per se crucial for the maintenance of apical-basal polarity in Drosophila melanogaster epithelial cells but rather regulated Myosin localization and phosphorylation. PATJ directly bound to the Myosin-binding subunit of Myosin phosphatase and decreased Myosin dephosphorylation, resulting in activated Myosin. Thereby, PATJ supports the stability of the Zonula Adherens. Notably, weakening of AJ in a PATJ mutant epithelium led first to a loss of Myosin from the AJ, subsequently to a disassembly of the AJ, and finally, to a loss of apical-basal polarity and disruption of the tissue.

PMID:
23128243
PMCID:
PMC3494860
DOI:
10.1083/jcb.201206064
[PubMed - indexed for MEDLINE]
Free PMC Article
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