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Photodermatol Photoimmunol Photomed. 2012 Dec;28(6):322-31. doi: 10.1111/phpp.12010.

Phloroglucinol protects human keratinocytes from ultraviolet B radiation by attenuating oxidative stress.

Author information

1
School of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju, Korea.

Abstract

BACKGROUND:

Ultraviolet B generates reactive oxygen species by interacting with water in irradiated skin tissues and cells in turn causes lipid peroxidation, protein modification and further DNA damage.

PURPOSE:

This study examined the cytoprotective effects of phloroglucinol (1,3,5-trihydroxybenzene) on ultraviolet B-irradiated cultured human keratinocytes.

METHODS:

The human keratinocyte cell line HaCaT cells were treated with 10 μM of phloroglucinol. After 1 h, the cells were irradiated with ultraviolet B light at 30 mJ/cm(2) and incubated at 37°C.

RESULTS:

Phloroglucinol scavenged both the superoxide anion and hydroxyl radical in a cell-free system and ultraviolet B-induced intracellular reactive oxygen species. Phloroglucinol reduced ultraviolet B-generated lipid peroxidation, protein modification and DNA strand breaks. The enzymatic effects of phloroglucinol restored cellular glutathione levels, superoxide dismutase and catalase activities, which were impaired by ultraviolet B radiation.

CONCLUSION:

Phloroglucinol provides the protective effects in human keratinocyte cell line exposed to ultraviolet B radiation, suggesting that phloroglucinol can be used as a photoprotective agent.

PMID:
23126295
DOI:
10.1111/phpp.12010
[Indexed for MEDLINE]

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