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Appl Environ Microbiol. 2013 Jan;79(2):718-21. doi: 10.1128/AEM.02926-12. Epub 2012 Nov 2.

Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria.

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1
Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, Virginia, USA.

Abstract

We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the P(BAD) promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacteria for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.

PMID:
23124231
PMCID:
PMC3553786
DOI:
10.1128/AEM.02926-12
[Indexed for MEDLINE]
Free PMC Article
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