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Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2012 Oct;37(10):973-8. doi: 10.3969/j.issn.1672-7347.2012.10.001.

[Effect of calcitonin gene-related peptide on the maturation of oocyte in vitro].

[Article in Chinese]

Author information

1
Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, China.

Abstract

OBJECTIVE:

To explore the effect of calcitonin gene-related peptide (CGRP) on murine oocyte maturation.

METHODS:

After injection of pregnant mare serum gonadotropin (PMSG, 10 U, i.p.) for 48 h, 6-week old female Kunming mice were killed, and the cumulus oocyte complexes (COCs) were collected from ovaries and inoculated in the culture plate by 30-40/hole. The COCs were treated with 4 concentrations of CGRP (0, 10(-10), 10(-9), and 10(-8) mol/L), and the germinal vesicle breakdown (GVBD) and polar body I (PBI) were examined. Human granulosa cells were also cultured with CGRP (0, 10(-10), 10(-9), 10(-8) mol/L) and levels of intracellular cyclic adenosine monophosphate (cAMP) were measured.

RESULTS:

Exogenous CGRP caused a decrease in GVBD and PBI in COCs, and an increase in cAMP levels in human granulosa cells in a concentration-dependent manner.

CONCLUSION:

CGRP can inhibit the oocyte maturation, which may be related to the increased content of cAMP in granulosa cells.

[Indexed for MEDLINE]
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