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J Vis Exp. 2012 Oct 18;(68). pii: 50032. doi: 10.3791/50032.

Skin tattooing as a novel approach for DNA vaccine delivery.

Author information

1
Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, USA.

Abstract

Nucleic acid-based vaccination is a topic of growing interest, especially plasmid DNA (pDNA) encoding immunologically important antigens. After the engineered pDNA is administered to the vaccines, it is transcribed and translated into immunogen proteins that can elicit responses from the immune system. Many ways of delivering DNA vaccines have been investigated; however each delivery route has its own advantages and pitfalls. Skin tattooing is a novel technique that is safe, cost-effective, and convenient. In addition, the punctures inflicted by the needle could also serve as a potent adjuvant. Here, we a) demonstrate the intradermal delivery of plasmid DNA encoding enhanced green fluorescent protein (pCX-EGFP) in a mouse model using a tattooing device and b) confirm the effective expression of EGFP in the skin cells using confocal microscopy.

PMID:
23117298
PMCID:
PMC3490318
DOI:
10.3791/50032
[Indexed for MEDLINE]
Free PMC Article
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