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Indian J Occup Environ Med. 2012 Jan;16(1):34-7. doi: 10.4103/0019-5278.99691.

Increase in DNA damage in lymphocytes and micronucleus frequency in buccal cells in silica-exposed workers.

Author information

1
Department of Genetics, Ramakrishna Mission Seva Pratishthan, Vivekananda Institute of Medical Sciences, 99 Sarat Bose Road, Kolkata, India.

Abstract

The alkaline single cell gel electrophoresis (comet assay) was applied to study the genotoxic properties of silica in human peripheral blood lymphocytes (PBL). The study was designed to evaluate the DNA damage of lymphocytes and the end points like micronuclei from buccal smears in a group of 45 workers, occupationally exposed to silica, from small mines and stone quarries. The results were compared to 20 sex and age matched normal individuals. There was a statistically significant difference in the damage levels between the exposed group and the control groups. The types of damages (type I -type 1V) were used to measure the DNA damage. The numbers of micronuclei were higher in the silica-exposed population. The present study suggests that the silica exposure can induce lymphocyte DNA damage and produces significant variation of micronuclei in buccal smear.

KEYWORDS:

Comet assay; lymphocyte DNA damage; micronuclei; silica-exposed female workers

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