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J Conserv Dent. 2012 Oct;15(4):351-6. doi: 10.4103/0972-0707.101902.

Comparative evaluation of effect of acidic beverage on enamel surface pre-treated with various remineralizing agents: An In vitro study.

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Department of Conservative Dentistry and Endodontics, A. B. Shetty Memorial Institute of Dental Sciences, Deralakatte, Mangalore, India.



This study aimed at quantitatively evaluating the effect of colabased beverage on the calcium loss of enamel surface pre-treated with fluorideenriched casein phosphopeptideamorphous calcium phosphate (CPP-ACPF) and βeta-ricalcium phosphate (β-TCP) using energy dispersive X-ray analysis (EDX).


24 enamel specimens were prepared from the buccal and palatal surfaces of extracted intact human premolars and were randomly assigned to study groups and control group. Specimens of Group II were pre-treated with CPP-ACPF and Group III treated with β-TCP twice daily for 4 for 28 days, followed by storage in artificial saliva. All specimens were evaluated for mineral (calcium and phosphorus) content (wt%) after pre-treatment using SEM-EDAX. The specimens were then placed in the acidic beverages for 4 days for 10 Mineral content was again measured using SEM-EDAX.


Statistical analysis using one-way ANOVA followed by Tukey's HSD test was applied to compare the re-mineralization and de-mineralization of the samples.


The present study concluded that both the remineralizing agents tested were found to be effective in inhibiting the de-mineralization caused by colabased beverage. Among the remineralizing agents tested, β-TCP was found to be more effective than CPP-ACPF.


Beta-ricalcium phosphate; CPP-ACPF; colabased beverage; de-mineralization; re-mineralization; scanning electron microscopy-energy dispersive X-ray analysis

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