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PLoS Biol. 2012;10(10):e1001411. doi: 10.1371/journal.pbio.1001411. Epub 2012 Oct 23.

Sequencing the connectome.

Author information

1
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America. zador@cshl.edu

Abstract

Connectivity determines the function of neural circuits. Historically, circuit mapping has usually been viewed as a problem of microscopy, but no current method can achieve high-throughput mapping of entire circuits with single neuron precision. Here we describe a novel approach to determining connectivity. We propose BOINC ("barcoding of individual neuronal connections"), a method for converting the problem of connectivity into a form that can be read out by high-throughput DNA sequencing. The appeal of using sequencing is that its scale--sequencing billions of nucleotides per day is now routine--is a natural match to the complexity of neural circuits. An inexpensive high-throughput technique for establishing circuit connectivity at single neuron resolution could transform neuroscience research.

PMID:
23109909
PMCID:
PMC3479097
DOI:
10.1371/journal.pbio.1001411
[Indexed for MEDLINE]
Free PMC Article

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