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Int J Mol Sci. 2012;13(9):11323-32. doi: 10.3390/ijms130911323. Epub 2012 Sep 11.

A novel collection of snRNA-like promoters with tissue-specific transcription properties.

Author information

1
Department of Experimental Medicine (DiMES), University of Genoa, 16132 Genoa, Italy; E-Mails: sgarritano@biologia.unipi.it (S.G.); arianna.gigoni@hotmail.it (A.G.); delfinacosta@gmail.com (D.C.); paolo.malatesta@unige.it (P.M.); ranieri.cancedda@unige.it (R.C.).

Abstract

We recently identified a novel dataset of snRNA-like trascriptional units in the human genome. The investigation of a subset of these elements showed that they play relevant roles in physiology and/or pathology. In this work we expand our collection of small RNAs taking advantage of a newly developed algorithm able to identify genome sequence stretches with RNA polymerase (pol) III type 3 promoter features thus constituting putative pol III binding sites. The bioinformatic analysis of a subset of these elements that map in introns of protein-coding genes in antisense configuration suggest their association with alternative splicing, similarly to other recently characterized small RNAs. Interestingly, the analysis of the transcriptional activity of these novel promoters shows that they are active in a cell-type specific manner, in accordance with the emerging body of evidence of a tissue/cell-specific activity of pol III.

KEYWORDS:

RNA polymerase III; alternative splicing; non-coding RNA; small nuclear RNA

PMID:
23109855
PMCID:
PMC3472747
DOI:
10.3390/ijms130911323
[Indexed for MEDLINE]
Free PMC Article

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