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Clin Immunol. 2012 Dec;145(3):189-200. doi: 10.1016/j.clim.2012.09.004. Epub 2012 Sep 21.

Human platelets can discriminate between various bacterial LPS isoforms via TLR4 signaling and differential cytokine secretion.

Author information

1
Université de Lyon, F-42023, GIMAP, EA3064, Saint-Etienne, France.

Abstract

Platelets are currently acknowledged as cells of innate immunity and inflammation and play a complex role in sepsis. We examined whether different types of LPS have different effects on the release of soluble signaling/effective molecules from platelets. We used platelet-rich plasma from healthy volunteers and LPS from two strains of gram-negative bacteria with disparate LPS structures. We combined LPS-stimulated platelet supernatants with reporter cells and measured the PBMC cytokine secretion profiles. Upon stimulation of platelets with both Escherichia coli O111 and Salmonella minnesota LPS, the platelet LPS::TLR4 interaction activated pathways to trigger the production of a large number of molecules. The different platelet supernatants caused differential PBMC secretion of IL-6, TNFα, and IL-8. Our data demonstrate that platelets have the capacity to sense external signals differentially through a single type of pathogen recognition receptor and adjust the innate immune response appropriately for pathogens exhibiting different types of 'danger' signals.

PMID:
23108090
DOI:
10.1016/j.clim.2012.09.004
[Indexed for MEDLINE]

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