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Nat Methods. 2012 Dec;9(12):1181-4. doi: 10.1038/nmeth.2214. Epub 2012 Oct 28.

Ultrabright photoactivatable fluorophores created by reductive caging.

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1
Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA.

Abstract

Sub-diffraction-limit imaging can be achieved by sequential localization of photoactivatable fluorophores, for which the image resolution depends on the number of photons detected per localization. We report a strategy for fluorophore caging that creates photoactivatable probes with high photon yields. Upon photoactivation, these probes can provide 10(4)-10(6) photons per localization and allow imaging of fixed samples with resolutions of several nanometers. This strategy can be applied to many fluorophores across the visible spectrum.

PMID:
23103881
PMCID:
PMC3561463
DOI:
10.1038/nmeth.2214
[Indexed for MEDLINE]
Free PMC Article
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