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Nat Methods. 2012 Dec;9(12):1195-7. doi: 10.1038/nmeth.2219. Epub 2012 Oct 28.

Phase-gradient microscopy in thick tissue with oblique back-illumination.

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1
Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA.

Abstract

Phase-contrast techniques, such as differential interference contrast microscopy, are widely used to obtain morphological images of unstained biological samples. The transillumination geometry required for these techniques restricts their application to thin samples. We introduce oblique back-illumination microscopy, a method of collecting en face phase-gradient images of thick scattering samples, enabling near-video-rate in vivo phase imaging with a miniaturized probe suitable for endoscopy.

PMID:
23103879
DOI:
10.1038/nmeth.2219
[Indexed for MEDLINE]
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