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Anal Biochem. 2013 Feb 15;433(2):92-4. doi: 10.1016/j.ab.2012.10.025. Epub 2012 Oct 24.

Fluorescent primer-based in vitro transcription system of viral RNA-dependent RNA polymerases.

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Unit of Viral Genome Regulation, Key Laboratory of Molecular Virology and Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025, People's Republic of China.


Viral infection is a leading cause of disease and death. Although vaccines are the most effective method of controlling viral infections, antiviral drugs are also important. Here, we established an in vitro transcription system by using fluorescein isothiocyanate-conjugated primers for RNA polymerases of viruses that are important disease-causing human pathogens (influenza, hepatitis C, Japanese encephalitis viruses, and enterovirus 71). This technology will allow us to analyze RNA polymerase activity without using radioisotopes.

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