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Biosens Bioelectron. 2013 Mar 15;41:783-8. doi: 10.1016/j.bios.2012.10.002. Epub 2012 Oct 8.

An electrochemical sensor based on label-free functional allosteric molecular beacons for detection target DNA/miRNA.

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1
Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Abstract

We report two novel electrochemical sensors (E-sensors) for the detection of target DNA and miRNA. The E-sensors were fabricated using label-free functional allosteric molecular beacons (aMBs), which can form streptavidin aptamers to bind to streptavidin peroxidase polymer and so generate catalytic currents in the presence of the targets. These E-sensors eliminate the antigen antibody interactions which require sophisticated DNA modification. During the experiment, we found a pair of CV peaks located at around 0.17 V. These peaks contributed to the redox reaction between TMB and TMB(+), and the adsorption-desorption process of TMB(+) to the negative aMB backbone. When the E-sensor was hybridized with the complement of the aMB sequence, a pair of CV peaks were found at around 0.47 V which were related to the redox reaction between TMB(+) and TMB(2+), and the process of intercalation of the planar structure of TMB(2+) to dsDNA. The RSV-aMB E-sensor could detect 44 amol RSV DNA in the 4 μL sample and performed well in complicated biological environments. The let-7a-aMB E-sensor reached a detection limit of 13.6 amol let-7a miRNA in the 4 μL sample and showed good selectivity for one base mismatched miRNA.

PMID:
23102830
DOI:
10.1016/j.bios.2012.10.002
[Indexed for MEDLINE]
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