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Indian J Microbiol. 2010 Sep;50(3):325-31. doi: 10.1007/s12088-010-0005-1. Epub 2010 Mar 5.

Influence of micronutrients on yeast growth and β-d-fructofuranosidase production.

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Department of Microbiology, Osmania University, Hyderabad, 500 007 India.


β-d-fructofuranosidase is one of the most important enzymes of the food industry especially due to its application in preparation of soft centered candies, confectioneries and to produce fructose syrups. Although several bacteria and filamentous fungi are reported for its production, yeasts are the most preferred source for this enzyme. In the present study mineral nutrients were screened for their effect on yeast growth and the enzyme production using Plackett-Burman design. Of the 11 nutrients screened, three variables (KH(2)PO(4), FeCl(3) and CoCl(2)) were significantly effecting yeast growth while six variables, i.e. KH(2)PO(4), CaCl(2), MnSO(4), Na(2)MoO(4), ZnSO(4) and CoCl(2) had significant effect on enzyme production.


Mineral nutrients; Plackett-Burman design; Saccharomyces species; Submerged fermentation; β-d-fructofuranosidase

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