Format

Send to

Choose Destination
See comment in PubMed Commons below
Stem Cells. 2013 Jan;31(1):215-20. doi: 10.1002/stem.1262.

Brief report: The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells.

Author information

1
Cell Therapy Program, Princess Margaret Hospital, University Health Network, Toronto, Ontario, Canada. gyannare@uhnres.utoronto.ca

Abstract

Human umbilical cord perivascular cells (HUCPVCs) are a readily available source of mesenchymal stromal cells (MSCs) for cell therapy. We were interested in understanding how differences from human bone marrow (BM)-derived MSCs might yield insights into MSC biology. We found that HUCPVCs exhibited increased telomerase activity and longer telomeres compared with BM-MSCs. We also observed enhanced expression of the pluripotency factors OCT4, SOX2, and NANOG in HUCPVCs. The methylation of OCT4 and NANOG promoters was similar in both cell types, indicating that differences in the expression of pluripotency factors between the MSCs were not associated with epigenetic changes. MSC methylation at these loci is greater than reported for embryonic stem cells but less than in dermal fibroblasts, suggesting that multipotentiality of MSCs is epigenetically restricted. These results are consistent with the notion that the MSC population (whether BM- or HUCPV-derived) exhibits higher proliferative capacity and contains more progenitor cells than do dermal fibroblasts.

PMID:
23097343
DOI:
10.1002/stem.1262
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center