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Angew Chem Int Ed Engl. 2013 Jan 2;52(1):331-5. doi: 10.1002/anie.201205474. Epub 2012 Oct 24.

β2-Adrenergic receptor solutions for structural biology analyzed with microscale NMR diffusion measurements.

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1
Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

Abstract

Microcoil NMR measurements were performed to determine the final composition of solutions of the β(2)-adrenergic receptor (β(2)AR) reconstituted with a detergent and to study the hydrodynamic properties of the detergent micelles containing β(2)AR. Standards are established for the reproducible preparation of G-protein-coupled receptor solutions for crystallization trials and solution NMR studies.

PMID:
23097212
PMCID:
PMC3729229
DOI:
10.1002/anie.201205474
[Indexed for MEDLINE]
Free PMC Article
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