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Nano Lett. 2012 Nov 14;12(11):5669-72. doi: 10.1021/nl302821g. Epub 2012 Nov 5.

Nanoblade delivery and incorporation of quantum dot conjugates into tubulin networks in live cells.

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1
Department of Chemistry & Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA.

Abstract

Quantum dots (QDs) have not been used to label cytoskeleton structure of live cells owing to limitations in delivery strategies, and QDs conjugation methods and issues with nonspecific binding. We conjugated tubulin to QDs and applied the emerging method of photothermal nanoblade to deliver QD-tubulin conjugates into live Hela cells. This method will open new opportunities for cytosolic targeting of QDs in live cells.

PMID:
23094784
PMCID:
PMC3500567
DOI:
10.1021/nl302821g
[Indexed for MEDLINE]
Free PMC Article
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