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Appl Phys Lett. 2012 Sep 10;101(11):113702.

Ultra-high cooling rate utilizing thin film evaporation.

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1
Institute of Marine Engineering and Thermal Science, Dalian Maritime University, Dalian 116026, China.

Abstract

This research introduces a cell cryopreservation method, which utilizes thin film evaporation and provides an ultra-high cooling rate. The microstructured surface forming the thin film evaporation was fabricated from copper microparticles with an average diameter of 50 μm. Experimental results showed that a cooling rate of approximately 5[Formula: see text]10(4) °C/min was achieved in a temperature range from 10 °C to -187 °C. The current investigation will give birth to a cell cryopreservation method through vitrification with relatively low concentrations of cryoprotectants.

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