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J Clin Immunol. 2013 Jan;33 Suppl 1:S57-61. doi: 10.1007/s10875-012-9814-9. Epub 2012 Oct 24.

Recurrent respiratory infections, specific antibody deficiencies, and memory B cells.

Author information

1
Department of Pediatrics, Allergy/Immunology Division, Louisiana State University Health Sciences Center, Jeffrey Modell Diagnostic Center for Primary Immunodeficiencies and Children's Hospital, 200 Henry Clay Avenue, New Orleans, LA 70118, USA. lleiva@lsuhsc.edu

Abstract

OBJECTIVES:

To investigate the immunological phenotypes detected in children with recurrent upper and lower respiratory infections that have normal total immunoglobulin concentrations.

METHODS:

A cohort of over 60 children with recurrent respiration infections was evaluated for specific antibody deficiencies (SAD) and for memory B-cell abnormalities. A control group of children without recurrent infections was also evaluated. Evaluation included a detailed history of immunizations with pneumococcal vaccines; determination of IgM, IgG, IgA, and IgE concentrations; measurement of anti-pneumococcal polysaccharide antibody levels by ELISA and expression of CD27, IgD, and IgM on peripheral CD19(+)B cells by flow cytometry to determine the proportions of naive, IgM-memory B cells, and class-switched memory B cells.

RESULTS:

Patients were classified as having a SAD to either pure polysaccharides (PPV-SAD) or to conjugate polysaccharides (PCV-SAD) based on the number of polysaccharides to which they developed an adequate antibody response. A normal response to only 2 or fewer of 7 PCV or PPV serotypes was considered as evidence of SAD. Forty-one patients without SAD and 26 with SAD were identified. IgM-memory B cells were low in 3 of 41 patients without SAD; in 3 of 5 PPV-SAD patients; and in 10 of 21 PCV-SAD patients. Class-switched memory B cells were low in 19 of 41 patients without SAD; in all 5 patients with PPV-SAD; and in 11 of 21PCV-SAD patients.

CONCLUSIONS:

Patients with recurrent infection with or without SAD may have low IgM- and/or class-switched memory B cells. Ongoing research aims to determine the prognostic implications of these differences in patients with SAD.

PMID:
23093309
DOI:
10.1007/s10875-012-9814-9
[Indexed for MEDLINE]
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